Fig. 2.

Effects of Ang II on the p130Cas protein assayed by Western blotting. (A) The bands for p130Cas protein at 130 kDa are reduced by 10^-7 M Ang II in a time-dependent manner. Such changes are further aggravated by compound C, a potent AMPK inhibitor. (B) At 24 hours, the density values for p130Cas decreased significantly at doses of 10^-7 M and 10^-6 M Ang II, respectively, compared to control (without Ang II) after correcting for β-tubulin levels. Data on the densitometric analysis of p130Cas/β-tubulin ratio are expressed as mean ± SD (n = 3). Control (100%); the value of no Ang II conditions. *P < 0.05 and ^† P < 0.01 vs. control.