Figure 1. Growth and characterization of MTS.
(A) Growth kinetics of MTS at 2, 4, 6 and 8 days of culture. ±SD n = 3, (B) Nuclei of cells of MTS at 2, 4, 6 and 8 days stained with DAPI (panel a, b, c and d respectively). (C) Six-day spheroids stained with H&E (a); and immunostaining with Ki67 (b); p27Kip1 (c); HIF1α (d). (D) Migration data monolayers vsspheroids disaggregated. The mean geometric diameter DG. Scale bar = 50 μm.