Table 1. Rates of oxygen consumption and OxPhos flux in MCF-7 monolayer and 6-day MTS.

	Monolayer cells	6-day MTS
Basal respiration (B.R.)a	5.2 ± 2	10.3 ± 2.7***
+5 μM oligomycin	1.7 ± 0.4	3 ± 0.4**
+1 mM NaCNb	0.6 ± 0.1	0.6 ± 0.3
OxPhos fluxc	3.5 ± 1.8	7.3 ± 2.5**
Uncoupled respiration (U)d	7.1 ± 1.1	17.2 ± 1.6**
U/B.R.	1.3 ± 0.1	1.7 ± 0.1*

All values are shown in ngAO/min/10⁶ cells. Data from five independent experiments are expressed as the mean ± SD. t- tests for paired data: *<0.05, **<0.005, ***<0.0005.

^aBasal respiration was determined in the absence of exogenous electron donors or carbon sources.

^bNaCN was added to full inhibit mitochondrial oxygen consumption.

^cOxPhos flux = Basal respiration rate - rate of oxygen consumption in the presence of oligomycin.

^dUncoupled respiration (U) was induced with 6 μM CCCP.