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. 2016 Mar 29;6:23533. doi: 10.1038/srep23533

Figure 1. The decrease in peripheral CD4+ T cells numbers in CD4-cre Runx1 cKO mice is not due to IL-7Rα mediated homeostasis.

Figure 1

(a) DP or CD4 SP thymocytes (left) and naïve CD4+ splenocytes (right) from WT or CD4-cre Runx1 cKO mice were examined for IL-7Rα expression. DP thymocytes are defined as CD4+ CD8+; CD4 SP thymocytes are defined as CD4+ CD8−; naïve CD4+ splenocytes are defined as CD4+ CD8−CD62L+ CD44−. Representative histograms are shown from five WT and six CD4-cre Runx1 cKO mice from five independent experiments. (b) Thymocytes and splenocytes from WT, CD4-cre Runx1 cKO, IL-7Rα tg, and IL-7Rα tg/CD4-cre Runx1 cKO were stained with CD4 and CD8 to examine T cell development. Data is representative from at least four mice in each group from five independent experiments. (c) Absolute numbers of DN, DP, CD4 SP, CD8 SP thymocytes, and (d) splenic CD4+ and CD8+ T cells are shown. Quantitation were generated from five WT, six CD4-cre Runx1 cKO, four IL-7Rα tg, and nine IL-7Rα tg/CD4-cre Runx1 cKO mice from five independent experiments. Error bars represent SEM.