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. Author manuscript; available in PMC: 2016 Mar 29.
Published in final edited form as: Nat Cell Biol. 2009 Mar 15;11(4):501–508. doi: 10.1038/ncb1858

Figure 2.

Figure 2

Depletion of rpS6 does not result in nucleolar disruption. (a, b) A549 cells treated with either NSsiRNA or rpS6 siRNA2 for 24 h or with 5 ng ml−1 actinomycin D (ActD) for 8 h were fixed and stained with anti-rpS6 and anti-NHP2 (a) or anti-rpS6 and anti-nucleolin (b) antibodies and analysed by confocal microscopy with a Plan-Apochromat 100×/1.4 numerical aperture Oil Dic objective (Zeiss; see Methods). Scale bar, 10 µm.