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. Author manuscript; available in PMC: 2016 Mar 29.
Published in final edited form as: Nat Cell Biol. 2009 Mar 15;11(4):501–508. doi: 10.1038/ncb1858

Figure 3.

Figure 3

Upregulation of p53 by depletion of ribosomal proteins is mediated by rpL11. (a) Western blots showing the levels of p53 and p21 in A549 cells transfected for 24 h with the indicated siRNAs. (b) Total RNA extracted from the indicated transfections, performed in parallel with those in a, were used to measure the levels of the rpS6 and rpL11 mRNAs by quantitative real-time PCR. Each bar represents the ratio of the indicated mRNA to that of β-actin mRNA, and results are shown as means ± s.e.m. for three independent transfection experiments. (c) BrdU incorporation in A549 cells transfected for 24 h with the indicated siRNAs. For each treatment the values are normalized to values for cells transfected with NSsiRNA. Results are shown as means ± s.e.m. for three independent experiments. (d) Polysome profiles from extracts of A549 cells treated for 24 h with either NSsiRNA (left panel) or siRNAs specific for rpS23 or rpL7a mRNAs. Insets: northern blots hybridized to probes specific for rpS23 and rpL7a mRNAs. (e) Western blots showing the levels of p53 and p21 proteins, and northern blots showing the levels of rpS6, rpS23, rpL7a and rpL11 mRNAs in A549 cells treated for 24 h with the indicated siRNAs. Uncropped images of blots are shown in Supplementary Information, Fig. S5.