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. Author manuscript; available in PMC: 2016 Mar 29.
Published in final edited form as: Bio Protoc. 2015 Oct 5;5(19):e1604. doi: 10.21769/bioprotoc.1604

Figure 3. Verification of the second PCR step in an EtBr-stained agarose gel (1.5%).

Figure 3

Lane 1: Molecular weight ladder. Lane 2: Total PCR sample (50 μl). Four bands of different size can be distinguished (A to D) that could be excised and purified as indicated in steps B6b-c.