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. 1999 Nov 1;104(9):R33–R39. doi: 10.1172/JCI7449

Figure 3.

Figure 3

EMSA analysis of binding activity to insulin promoter. (a) EMSA analysis of IPF-1–binding activity in nuclear extracts prepared from untransfected Nes2y cells (lane 1), cells expressing normal IPF-1 (lane 2), C18R (lane 3), D76N (lane 4), or R197H (lane 5), using the A3 site of the human insulin gene promoter as a probe. The upper band (arrow) represents IPF-1–binding activity. An analysis of 0.5 μg of each sample was performed, and the results are representative of 3 separate experiments. (b) EMSA analysis of USF-binding activity in nuclear extracts prepared from untransfected Nes2y cells (lane 1) or from cells expressing normal IPF-1 (lane 2), C18R (lane 3), D76N (lane 4), or R197H (lane 5), using the E2 site of the human insulin gene promoter as a probe. An analysis of 0.5 μg of each sample was performed, and the results are representative of 3 separate experiments.