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. 1999 Nov 1;104(9):R33–R39. doi: 10.1172/JCI7449

Figure 4.

Figure 4

Analysis of the transcriptional activation of the insulin gene in Nes2y cells. Relative luciferase activity from control construct pGL-Luc (LUC) or from a construct containing the –60 to –260 region of the human insulin gene promoter pGL-Luc200 (LUC200) in 3 mM (open bars) or 20 mM (filled bars) glucose. Reporter gene activity was analyzed in untransfected Nes2y cells (a), or in Nes2y cells expressing normal IPF-1 (b), C18R (c), D76N (d), or R197H (e). Values are shown as relative luciferase activity standardized against protein content. Values represent an average from 6 replicates; error bars represent SD. Each set of values has been reproduced in 3 separate experiments.