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. 2016 Mar 1;36(6):965–978. doi: 10.1128/MCB.00773-15

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FIG 3 — Utp14 mutants show reduced 27SA2 pre-rRNA. (A) Cartoon for rRNA processing. (B and C) Northern blots: P_GAL1-Utp14 (AJY3243) containing empty vector (pAJ100) (lane 1), wild-type UTP14 (pAJ1919) (lane 2), utp14_multi-Sup(pAJ3264) (lane 3), utp14_multi-Ala(pAJ3276) (lane 4), and utp14_Δ_719-780 (pAJ3276) (lane 5) were grown in SD Ura⁻ galactose medium and then shifted to SD Ura⁻ glucose medium for 6 h at 30°C. All cultures were harvested at an OD₆₀₀ of ∼0.3. Total RNA was extracted using hot phenol, separated on a 1% agarose-formaldehyde denaturing gel, transferred to a membrane, and probed with probe 1 (AJO603, site A2-A3) (B) and probe 2 (AJO130, site D-A2) (C). U2 RNA (probed with AJO962) was used as a loading control.