FIG 4.
Dhr1 sedimentation in utp14 mutants. Whole-cell extracts were prepared from a PGAL1-Utp14 strain (AJY3243) containing plasmids expressing wild-type UTP14 (pAJ3308) (A), utp14multi-Sup (pAJ3309) (B), utp14multi-Ala (pAJ3313) (C), and utp14Δ719-780 (pAJ3310) (D) grown in glucose medium for 6 h. Extracts were subjected to sucrose density gradient ultracentrifugation. Proteins were precipitated from fractions and subjected to Western blotting, and RNA was extracted and subjected to Northern blotting. Utp14, Dhr1, Imp4, and Mpp10 were detected using anti-GFP (Utp14), anti-myc (Dhr1), anti-Imp4, and anti-Mpp10 antibodies, respectively. U3 was probed with AJO2194.