FIG 9.
Utp14 and Bud23 together are necessary for efficient Dhr1 recruitment to the preribosome. Plasmid pAJ3081 (dhr1K420A-13myc) was transformed into wild-type (BY4741) (A), bud23Δ (AJY2161) (B), PGAL1-Utp14 (AJY3243) (C), and PGAL1-Utp14 bud23Δ (AJY3245) (D) strains. Whole-cell extracts from strains grown in Leu− galactose medium that were shifted to Leu− glucose medium for 6 h were subjected to sucrose density gradient ultracentrifugation. Proteins were precipitated from fractions and subjected to Western blotting. Dhr1K420A was detected with anti-myc antibody.