TABLE 2.
Plasmids used in this work
| Plasmid | Description | Source or reference |
|---|---|---|
| pACT2 | GAL4AD-HA LEU2 2μ | Clontech |
| pAJ1918 | utp14A758G URA3 CEN ARS | 23 |
| pAJ1919 | UTP14 URA3 CEN ARS | 23 |
| pAJ2311 | DHR1-13myc LEU2 CEN ARS | 24 |
| pAJ2312 | pET21a-DHR1-6×His | 18 |
| pAJ2321 | GAL4AD-HA-UTP14 LEU2 2μ | This study |
| pAJ2330 | GAL4BD-c-myc-dhr11–838 TRP1 2μ | This study |
| pAJ2331 | GAL4BD-c-myc-dhr1319–838 TRP1 2μ | This study |
| pAJ2332 | GAL4BD-c-myc-dhr1319–1267 TRP1 2μ | This study |
| pAJ2334 | GAL4AD-HA-utp141–706 LEU2 2μ | This study |
| pAJ2335 | GAL4AD-HA-utp14707–899 LEU2 2μ | This study |
| pAJ2341 | GAL4AD-HA-utp141–813 LEU2 2μ | This study |
| pAJ2342 | GAL4AD-HA-utp141–654 LEU2 2μ | This study |
| pAJ2343 | GAL4AD-HA-utp141–564 LEU2 2μ | This study |
| pAJ2344 | GAL4AD-HA-utp141–265 LEU2 2μ | This study |
| pAJ2345 | GAL4AD-HA-utp14266–899 LEU2 2μ | This study |
| pAJ2346 | GAL4AD-HA-utp14565–899 LEU2 2μ | This study |
| pAJ2347 | GAL4AD-HA-utp14655–899 LEU2 2μ | This study |
| pAJ2394 | LexABD-c-myc-DHR1 TRP1 2μ | This study |
| pAJ2593 | DHR1 URA3 CEN ARS | 24 |
| pAJ2795 | GAL4AD-HA-dhr1467–1267 LEU2 2μ | 24 |
| pAJ2796 | GAL4AD-HA-dhr11–142 LEU2 2μ | 24 |
| pAJ2797 | GAL4AD-HA-dhr11–467 LEU2 2μ | 24 |
| pAJ2922 | GAL4BD-c-myc-DHR1 | 24 |
| pAJ3081 | dhr1K420A-13myc LEU2 CEN ARS | 18 |
| pAJ3132 | utp14I755T URA3 CEN ARS | This study |
| pAJ3136 | utp14E757G URA3 CEN ARS | This study |
| pAJ3145 | utp14V754G URA3 CEN ARS | This study |
| pAJ3145 | utp14A760P URA3 CEN ARS | This study |
| pAJ3263 | GAL4AD-HA-utp14D753A/V754A/I755A/E757A/F759A LEU2 2μ | This study |
| pAJ3264 | utp14V754G/I755T/E757G/A758G/A760P URA3 CEN ARS | This study |
| pAJ3265 | GAL4AD-HA-utp14Δ719–780 LEU2 2μ | This study |
| pAJ3266 | GAL4AD-HA-utp14V754G/I755T/E757G/A758G/A760P LEU2 2μ | This study |
| pAJ3267 | utp14Δ719–780 URA3 CEN ARS | This study |
| pAJ3276 | utp14D753A/V754A/I755A/E757A/F759A URA3 CEN ARS | This study |
| pAJ3307 | pET16a-6×His-UTP14 | This study |
| pAJ3308 | UTP14-GFP URA3 CEN ARS | This study |
| pAJ3309 | utp14V754G/I755T/E757G/A758G/A760P-GFP URA3 CEN ARS | This study |
| pAJ3310 | utp14D753A/V754A/I755A/E757A/F759A-GFP URA3 CEN ARS | This study |
| pAJ3313 | utp14Δ719–780M-GFP URA3 CEN ARS | This study |
| pAJ3314 | pET16a-6×His-utp14Δ719-780 | This study |
| pAJ3315 | pET16a-6×His-utp14D753A/V754A/I755A/E757A/F759A | This study |
| pAJ3331 | 6×His-UTP14 URA3 CEN ARS | This study |
| pAJ3421 | utp141–813 URA3 CEN ARS | This study |
| pAJ3422 | utp141–706 URA3 CEN ARS | This study |
| pAJ3423 | utp141–654 URA3 CEN ARS | This study |
| pAJ3424 | utp141–564 URA3 CEN ARS | This study |
| pAJ3425 | utp141–265 URA3 CEN ARS | This study |
| pAJ3426 | utp14266–899 URA3 CEN ARS | This study |
| pAJ3427 | utp14565–899 URA3 CEN ARS | This study |
| pAJ3428 | utp14655–899 URA3 CEN ARS | This study |
| pAJ3429 | utp14707–899 URA3 CEN ARS | This study |
| pAJ3455 | dhr1319–1267 URA3 CEN ARS | This study |
| pAJ3456 | dhr1467–1267 URA3 CEN ARS | This study |
| pAJ3457 | dhr11–838 URA3 CEN ARS | This study |
| pAJ3458 | dhr11–142 URA3 CEN ARS | This study |
| pAJ3459 | dhr11–467 URA3 CEN ARS | This study |
| pAJ3460 | GAL4BD-c-myc-dhr1584–1267 TRP1 2μ | This study |
| pAJ3461 | GAL4BD-c-myc-dhr1839–1267 TRP1 2μ | This study |
| pAJ3470 | dhr1319–838 URA3 CEN ARS | This study |
| pGBKT7 | GAL4BD-c-myc TRP1 2μ | Clontech |