TABLE 1 .

Table listing eight orthologous groups of proteins from Rhodococcus jostii RHA1, Mycobacterium tuberculosis H37Rv, and Comamonas testosteroni CNB-2 used as references for HMM generation^a

RHA1/H37Rv name	CNB-2 name	CNB-2 testosterone/cholate cluster	RHA1cholate cluster	RHA1cholesterol cluster	H37Rvcholesterol cluster	Annotation
KstD	TesH	CtCNB1_1357	RHA1_ro05798	RHA1_ro04532	Rv3537	3-Ketosteroid-dehydrogenase
		(CTCNB1_RS06925)	(RHA1_RS28305)	(RHA1_RS22090)
			RHA1_ro05813
			(RHA1_RS28380)
KshA	No name	CtCNB1_1306	RHA1_ro05811	RHA1_ro04538	Rv3526	3-Ketosteroid-9-alpha hydroxylase (oxygenase)
		(CTCNB1_RS06665)	(RHA1_RS28370)	(RHA1_RS22120)
HsaA	TesA2	CtCNB1_1356	RHA1_ro05802	RHA1_ro04539	Rv3570c	3-Hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione-4-hydroxylase (oxygenase)
		(CTCNB1_RS06920)	(RHA1_RS28325)	(RHA1_RS22125)
HsaC	TesB	CtCNB1_1275	RHA1_ro05803	RHA1_ro04541	Rv3568c	3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione-4,5-dioxygenase
		(CTCNB1_RS06510)	(RHA1_RS28330)	(RHA1_RS22135)
HsaD	TesD	CtCNB1_1354	RHA1_ro05797	RHA1_ro04540	Rv3569c	4,5-9,10-Diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
		(CTCNB1_RS06910)	(RHA1_RS28300)	(RHA1_RS22130)
HsaE	TesE	CtCNB1_1353	RHA1_ro05799	RHA1_ro04533	Rv3536c	2-Hydroxyhexa-2,4-dienoate hydratase
		(CTCNB1_RS06905)	(RHA1_RS28310)	(RHA1_RS22095)
HsaF	TesG	CtCNB1_1351	RHA1_ro05801	RHA1_ro04535	Rv3534c	4-Hydroxy-2-oxohexanoate aldolase
		(CTCNB1_RS06905)	(RHA1_RS28320)	(RHA1_RS22105)
HsaG	TesF	CtCNB1_1352	RHA1_ro05800	RHA1_ro04534	Rv3535c	Propanol dehydrogenase
		(CTCNB1_RS06900)	(RHA1_RS28315)	(RHA1_RS22100)

^aLocus tags of the respective proteins are listed as used in this study and after subsequent GenBank reannotation (the latter are indicated in parentheses).