FIG 6 .

The pUL136 isoforms differentially influence the establishment of latency and virus reactivation in CD34⁺ HPCs. CD34⁺ HPCs were infected at an MOI of 2 with the indicated virus and sorted by FACS to isolate pure, infected populations. HPCs were maintained in LTBMC over stromal cell support for 10 days. Subsequently, HPCs were cocultured with an MRC-5 fibroblast cell monolayer (mock reactivation). In parallel, lysates from an equal number of HPCs were plated with an MRC-5 fibroblast cell monolayer (prereactivation). At 14 days later, 96-well dishes were scored for GFP⁺ wells, and the frequency of infectious centers was determined using ELDA software. Statistical significance was determined using two-way analysis of variance followed by Dunnett’s posttest for comparisons to UL136_myc virus results. P values are shown.