FIG 6 .
Chimeric BvgS variants with natural linkers of homologs. (A) Sequences of the variants in which the region between the TM segment and the DHp domain of BvgS was replaced with natural linkers of PAS-less BvgS homologs. The species from which the sequences originate are indicated, as follows: Pb, Pseudomonas brassicacearum (GI:742884217); Pf, Pseudomonas fragi (GI:515153750); Pp, Pseudomonas putida (GI:24985059); and Ec, Enterobacter cloacae (GI:915610361). The variants obtained were named ΔPAS-R1, -R2, -R3, and -R4, respectively. The first 4 residues of the DHp domain originate from the respective homolog, followed by the BvgS sequence from the FLAT motif on. (B) The ptx-lacZ reporter was used to determine the basal activities and the responses to nicotinate (Nico) and chloronicotinate (CN) (at the indicated millimolar concentrations) of the BvgSΔPAS variants compared to those of wt BvgS (WT). The means and standard errors of the means are given. nd, not determined.