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Immunohistochemistry of myotubes from primary cell culture from C57 and mdx muscle. The presence of dystrophin was detected by mAb to the COOH-terminus of dystrophin (F192A12) followed by a rhodamine-conjugated anti-mouse IgG. Dystrophin was present in myotubes from C57 mice (left) and in mdx myotubes treated with 300 μg/mL (middle). No dystrophin was detected in untreated mdx myotubes (right).
