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. 2016 Mar 28;16:23. doi: 10.1186/s12935-016-0297-2

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© Chang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — HRM assays and sequence traces for APC exon 4. a Difference plot showed two different melting profiles, wild-type (WT) samples was blue, mutation in other colors. Direct DNA sequencing confirmed the b WT and the presence of the APC exon 4 mutation: c c.465A>G