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Images of ice in the presence of RBCs with (A) 15% glycerol, (B) 30 mM 4 in 15% glycerol and (C) 5 mM 5 in 15% glycerol. Samples were cooled to −40 °C (25 °C/min) and then warmed (10 °C/min) to −20 °C. Images shown are after 10 minutes at this temperature. Mean grain size and percentage of ice in the sample is smaller when IRI 4 (30 mM) (B) or 5 (5 mM) (C) is present in the 15% glycerol solution compared to the 15% glycerol control (A).
