TABLE 1.
HLA-A*02:01-restricted epitopes selected from HSV glycoproteinsa
| Antigen and peptide | Sequence | MW | No. (aa) | HLA-A*0201 (IC50 [nM]) |
|---|---|---|---|---|
| gD | ||||
| gD-18 to –10 | RLGAVILFV | 987 | 9 | 62 |
| gD-13 to –5 | ILFVVIVGL | 972 | 9 | >50,000 |
| gD-11 to –2 | AVVIVGLHGV | 963 | 10 | >50,000 |
| gD53-61 | SLPITVYYA | 1,026 | 9 | 20 |
| gD70-78 | VLLNAPSEA | 913 | 9 | 19 |
| gD95-103 | NLTIAWPRM | 1,101 | 9 | >50,000 |
| gD153-161 | LMHAPAFET | 1,016 | 9 | 2,002 |
| gD224-232 | FIPENQRTV | 1,053 | 9 | 4,218 |
| gD253-261 | LLPPELSET | 998 | 9 | 4,891 |
| gD278-286 | ALLEDPVGT | 916 | 9 | 28 |
| gB | ||||
| gB17-25 | ALLGLTLGV | 856 | 9 | 1.3 |
| gB183-191 | GIFEDRAPV | 1,003.1 | 9 | |
| gB286-294 | FVLATGDFV | 968.1 | 9 | |
| gB342-350 | NLLTTPKFT | 1,034.2 | 9 | |
| gB343-351 | LLTTPKFTV | 1,019.2 | 9 | |
| gB441-449 | YLANGGFLI | 967.1 | 9 | 0.56 |
| gB447-455 | FLIAYQPLL | 1,077.3 | 9 | 0.31 |
| gB561-569 | RMLGDVMAV | 991.2 | 9 | 1.7 |
| gB675-683 | TMLEDHEFV | 1,120.2 | 9 | 118 |
a
The sequence ofHSV-1 gD and gB was subjected to screening of potential HLA-A*02:01 epitopesusing several computer algorithms. Ten peptides were selected on the basis ofthe HLA-A*0201 binding motif sequence from HSV-1 strain 17. Immunodominant epitopes are indicated in boldface. MW, molecular weight; aa, amino acids.