FIG 2.

Binding of PSV to synthetic HBGAs. The binding ability of PSV strains (KS05151, KS05152, KS04105, or KS055217), the P domain of the human NV VA207 (GII.9) and VA387 (GII.4) strains, VP8* of RV strain Dhaka6, and influenza virus A/PR/8/34 (H1N1) were determined using HRP-conjugated streptavidin. Binding of HBGA was visualized using TMB and measured at 450 nm in three independent experiments. Error bars represent SD. The samples with absorbance greater than 0.62 of the cutoff value, indicated by red dash line (3 standard deviations above the mean OD of the wells with negative control H1N1 influenza virus), were considered positive. The mean ODs in the no-HBGA control and no-antigen control wells as a background are 0.04 and 0.06, respectively.