TABLE 1.
BAC-cloned HCMV strains and derivatives used
| HCMV strain | Name | GenBank accession no. | Reference | UL128L origin and nucleotide featuresb | Designation of derived virus |
|---|---|---|---|---|---|
| BAC-cloned strainsa | |||||
| Merlin | Merlin-BAC | GU179001.1 | 15 | Wild-type | Merlin-UL128Lwt |
| G>A in UL128 at nt 176260 (R>stop) | Merlin-UL128Lmut | ||||
| TR | TR-BAC | AC146906.1 | 45 | Intact | TR |
| TB40/E | TB40-BAC4 | EF999921.1 | 47 | Intact | TB40 |
| VR1814 | FIX-BAC | AC146907.1 | 48 | Intact | FIX |
| Clinical strain (nonpassaged) | |||||
| 3301 | GQ466044.1 | 41 | Wild type | 3301 | |
| Merlin derivatives | |||||
| Containing UL128L of other strains | 23 | 3301 | Merlin-UL128L3301 | ||
| TR | Merlin-UL128LTR | ||||
| TB40-BAC4 | Merlin-UL128LTB40 | ||||
| FIX | Merlin-UL128LFIX | ||||
| Containing single nucleotides from UL128L of other strains | 23 | TB40-BAC4 G>T in UL128 at nt 176612 (intron) | Merlin-UL128G>T | ||
| FIX-BAC A>G in UL130 at nt 177364 (S>P) | Merlin-UL130A>G |
a
GenBank accession numbers for the BAC-cloned parental strains are as follows: Merlin, NC_006273.2 (74); TR, KF021605.1 (46); VR1814, GU179289.1 (9). An accession number for strain TB40/E (7) is not available.
b
Nucleotide positions are relative to the sequence of BAC-cloned HCMV strain Merlin (GenBank accession number GU179001). nt, nucleotide.