TABLE 3.
Mutant populations viruses generated by 5 passages in fibroblast (HFFF) cells
| Virus | Mutation location (nt)a | ORF(s) affected | Mutation and/or coding effect | Mutant population (% of sequenced population) |
|---|---|---|---|---|
| Merlin-UL128Lmut | No de novo mutations detected | |||
| Merlin-UL128Lwt | 176320–187358 | UL128–UL139 | 11,039-bp deletion | 97 |
| 178063–178066 | UL131A | 4-bp deletion; frameshift | 3 | |
| Merlin-UL128L3301 | 228878–446 | UL128 exon 1 | G insertion; frameshift | 64 |
| 81884 | UL54/UL55 IG | G>A substitution | ||
| TR | 194202-BAC | a′-US3 | 6,087-bp deletion: 3,899 bp from a′ to the BAC vector, plus 2,188 bp of the BAC | 92 |
| 4,306-bp deletion: 3,899 bp from a′ to the BAC vector, plus 407 bp of the BAC | 92 | |||
| TB40-BAC4 | 228878–446 | US7 | Deletion of 173 bp from a′ to the BAC vector, entire BAC vector, and 446 bp into US7 | 6 |
| FIX | 228002–60 | 7,650-bp deletion; sequence from a′ to BAC vector plus 7 bp | 100 | |
| Merlin-UL128LTB40 | 177130–179950 | UL130–UL148 | 2,807-bp deletion | 54 |
| NA | eGFP | T>A substitution | 61 | |
| Merlin-UL128G>T | 61647 | UL47 | G>C (D>H) substitution | 29 |
| 83915 | UL55 | T>A (D>V) substitution | 11 | |
| Merlin-UL128LFIX | No de novo mutations detected | |||
| Merlin-UL130A>G | NA | eGFP | 720-bp deletion | 99 |
a
Locations are based on the sequences of TR-BAC (GenBank accession number AC146906.1), TB40-BAC4 (GenBank EF999921.1), and Merlin-BAC (GenBank GU179001.1). The TR-BAC, TB40-BAC4, and FIX-BAC sequences deposited in GenBank commence immediately following the site of vector insertion, and coordinates of mutations in viruses derived from these clones describe sequences either side of the BAC vector. NA, not available; nt, nucleotide.