FIG 5.
(A) Effect of a JFH-1 core mutant deficient in LD-targeting on CIDEB cleavage. Western blot analysis of Huh-7.5 cells was performed 48 h after electroporation of cells with 10 μg of wild-type JFH-1 (WT) or JFHDP RNA. (B) Localization of endogenous CIDEB and HCV core after HCV infection assayed by LD fractionation. Fifteen micrograms of whole-cell lysate and 7.5 μg of purified LD-associated proteins were analyzed by Western blotting. (C) Colocalization of exogenously overexpressed FLAG-tagged CIDEB and HCV core protein on lipid droplets.