FIG 6.

CIDEB modulates LD stability. (A) LD stability in naive or JFH-1/AD16-infected Huh-7.5 cells. Cells were incubated in lipid-rich medium for 14 h, washed with PBS, and cultured in DMEM with 5.5 μM TriC for 24 h before staining with ORO (red, 100×) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) or with anti-NS3 (green, 40×) and DAPI. (B) Formation of LDs in Huh-7.5 or Huh-7.5 CIDEB-KO cells after lipid loading. Cells were incubated in lipid-rich medium for 20 h, washed with PBS, and stained for LDs using ORO (red, 100×). (C) LD stability in wild-type and CIDEB KO cells. After lipid loading as described above, cells were cultured in DMEM with or without 5.5 μM TriC for 24 h and then stained for LDs using ORO (red, 100×). Panels 1, 2, and 3 are separate frames. DAPI (blue) was used as a counterstain.