FIG 3.

Only the 5′ part of the intron is required for RIS-Ad-mediated inhibition of adenoviral replication. (A, C, and E) Schematic representation of the variants with 3′ deletions of the Rep1701/2186 sequence (RIS-Ad) (A), variants with both 5′ and 3′ deletions (C), and domain swap constructs (E), all of which were assayed for inhibition of adenoviral replication. (A and C) Boxes with different shading and open boxes represent the p40 promoter with defined transcription factor-binding sites, the 3′ nontranslated region of the cap transcripts (nt 1853 to 1906), and the intron sequence. (E) Different shaded and open boxes represent the wild-type and recoded (sRep) p40 promoter, the 3′ nontranslated cap region, and the AAV-2 intron sequences. The luciferase ORF is also indicated. (B, D, and F) Amounts of recombinant adenoviral particles determined in freeze-thaw cell supernatants after transfection and first-round amplification of the indicated AdEasy constructs as described in the legend to Fig. 1C.