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. 2016 Mar 28;90(8):4160–4173. doi: 10.1128/JVI.02834-15

FIG 5.

FIG 5

SEGS-2 enhances CMB infection in N. benthamiana. (A) N. benthamiana plants inoculated with EACMV-UG DNA-A plus DNA-B showed no symptoms, while plants coinoculated with EACMV-UG DNA-A plus DNA-B and SEGS-2 were symptomatic at 14 dpi. (B) Plants inoculated with EACMCV DNA-A plus DNA-B showed very mild symptoms, while those coinoculated with EACMCV DNA-A plus DNA-B and SEGS-2 displayed strong symptoms at 14 dpi. (C) Plants inoculated with SEGS-2 alone did not develop symptoms. (D) Total DNA was extracted at 14 dpi from systemically infected leaves and equivalent leaves from symptom-free plants and analyzed by DNA gel blotting with radiolabeled probes corresponding to EACMV-UG DNA-A (lanes 1 to 4) or EACMCV DNA-A (lanes 5 to 8). The lanes correspond to mock inoculation (M; lanes 1 and 5), CMB alone (lanes 2 and 5), CMB plus SEGS-2 (lanes 3 and 7), and SEGS-2 alone (lanes 4 and 8). The loading control is ethidium-stained, total genomic DNA.