FIG 1.

Characterization of the ac54-knockout bacmid. (A) Strategy for the construction of the recombinant viruses vAc54KO, vAc54:2HA, and vAcWT. (B) Sf9 cells were transfected with vAc54KO, vAc54:2HA, and vAcWT bacmid DNA and observed at 72 h p.t. (C) Virus growth curves. The supernatants of transfected cells were harvested at different time points, and viral titers were determined by TCID₅₀ endpoint dilution assays. Each data point represents the average titer of three independent biological repeats; error bars indicate standard deviations. (D) Real-time PCR analysis of viral DNA synthesis in Sf9 cells. Total DNA was extracted from Sf9 cells transfected with vAc54KO, vIE1KO, or v38KKO bacmid DNA at indicated time points, further digested with the restriction enzyme DpnI to eliminate input bacmid DNA, and quantified by real-time PCR. Data are shown as mean values ± standard deviations.