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. 2016 Mar 28;90(8):4115–4126. doi: 10.1128/JVI.02885-15

FIG 4.

FIG 4

Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA. At 36 h p.t., the cells were fixed, incubated with an anti-β-actin antibody and visualized with Alexa Fluor 647-conjugated donkey anti-mouse antibody (red). Hoechst 33342 was used to visualize DNA-rich regions of the nucleus (blue). (B) F-actin localization. Sf9 cells were infected with AcMNPV or transfected with bAc54KO, bMON14272 (indicated as WT in the figure), b38KKO, or bPP78/83KO bacmid DNA. At 36 h p.i. or 48 h p.t., cells were fixed and stained with both phalloidin-FITC (red) and Hoechst 33342 (blue).