TABLE 1.
Primer | Sequencea | Restriction enzyme |
---|---|---|
ac54-CmF | 5′ -GATCAAGCCGAGGCGAGAAAAACAATTCTCAACGCCGACAGCGTGTACGAGTGCATGTTAATTAGACCAATTCGTACGTAAAATACCTGTGACGGAAGA | |
ac54-CmR | 5′-ATCCGCCGCCGGTTTCTGATTGGCGCGCGATTAACTCGCTGTATCGTTTGTACTTGTTGGGATTTCGAACCCACTTTGCTAAACCAGCAATAGACATAA | |
CmUS | 5′-TAAAATACCTGTGACGGAAGA | |
CmDS | 5′-TAAACCAGCAATAGACATAA | |
ac54US | 5′-ATGTGTTCGACCAAGAAACC | |
ac54DS | 5′- CTACACGTTGTGTGCGTGCA | |
REPac54US | 5′-TCTAGAGAGATCCCTTTAATCGTGT | XbaI |
REPac54DS | 5′-CTGCAGCTAAGCGTAATCTGGAACATCGTATGGGTAAGCGTAATCTGGAACATCGTATGGGTACACGTTGTGTGCGTGCAGAC | PstI |
SV40DS | 5′-GAGCTCGATCCAGACATGATAAGATA | SacI |
ac54US-T.E. | 5′-GGATCCGAGATCCCTTTAATCGTGT | BamHI |
ac54DS-T.E. | 5′-GATATCCACGTTGTGTGCGTGCAG | EcoRV |
ac54HAUS-T.E. | 5′-GGATCCGCCACCATGTGTTCGACCAAGAAACCG | BamHI |
ac54HADS-T.E. | 5′-GATATCTTAGGCGTAGTCGGGCACGTCGTAGGGGTACACGTTGTGTGCGTGCAGACCAA | EcoRV |
c42US-T.E. | 5′-GGATCCATGGCCACCAGCGCTATCGCGTTGTAT | BamHI |
c42DS-T.E. | 5′-CTCGAGTCACTTATCGTCGTCATCCTTGTAATC ATATTTTTTACGCTTTGC | XhoI |
vp39 US-T.E. | 5′-GGATCCATGGCCACCGCGCTAGTGCCCGTGGGT | BamHI |
vp39 DS-T.E. | 5′-CTCGAGTCACTTATCGTCGTCATCCTTGTAATC GACGGCTATTCCTCCACC | XhoI |
vp80 US-T.E. | 5′-GGATCCATGGCCACCAACGATTCCAATTCTCTG | BamHI |
vp80 DS-T.E. | 5′-CTCGAGTCACTTATCGTCGTCATCCTTGTAATC TATAACATTGTAGTTTGC | XhoI |
Underlining indicates sequence complementary to the chloramphenicol resistance gene; boldface indicates restriction enzyme sites.