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. 2016 Mar 28;90(8):4115–4126. doi: 10.1128/JVI.02885-15

TABLE 1.

List of primers used in this paper

Primer Sequencea Restriction enzyme
ac54-CmF 5′ -GATCAAGCCGAGGCGAGAAAAACAATTCTCAACGCCGACAGCGTGTACGAGTGCATGTTAATTAGACCAATTCGTACGTAAAATACCTGTGACGGAAGA
ac54-CmR 5′-ATCCGCCGCCGGTTTCTGATTGGCGCGCGATTAACTCGCTGTATCGTTTGTACTTGTTGGGATTTCGAACCCACTTTGCTAAACCAGCAATAGACATAA
CmUS 5′-TAAAATACCTGTGACGGAAGA
CmDS 5′-TAAACCAGCAATAGACATAA
ac54US 5′-ATGTGTTCGACCAAGAAACC
ac54DS 5′- CTACACGTTGTGTGCGTGCA
REPac54US 5′-TCTAGAGAGATCCCTTTAATCGTGT XbaI
REPac54DS 5′-CTGCAGCTAAGCGTAATCTGGAACATCGTATGGGTAAGCGTAATCTGGAACATCGTATGGGTACACGTTGTGTGCGTGCAGAC PstI
SV40DS 5′-GAGCTCGATCCAGACATGATAAGATA SacI
ac54US-T.E. 5′-GGATCCGAGATCCCTTTAATCGTGT BamHI
ac54DS-T.E. 5′-GATATCCACGTTGTGTGCGTGCAG EcoRV
ac54HAUS-T.E. 5′-GGATCCGCCACCATGTGTTCGACCAAGAAACCG BamHI
ac54HADS-T.E. 5′-GATATCTTAGGCGTAGTCGGGCACGTCGTAGGGGTACACGTTGTGTGCGTGCAGACCAA EcoRV
c42US-T.E. 5′-GGATCCATGGCCACCAGCGCTATCGCGTTGTAT BamHI
c42DS-T.E. 5′-CTCGAGTCACTTATCGTCGTCATCCTTGTAATC ATATTTTTTACGCTTTGC XhoI
vp39 US-T.E. 5′-GGATCCATGGCCACCGCGCTAGTGCCCGTGGGT BamHI
vp39 DS-T.E. 5′-CTCGAGTCACTTATCGTCGTCATCCTTGTAATC GACGGCTATTCCTCCACC XhoI
vp80 US-T.E. 5′-GGATCCATGGCCACCAACGATTCCAATTCTCTG BamHI
vp80 DS-T.E. 5′-CTCGAGTCACTTATCGTCGTCATCCTTGTAATC TATAACATTGTAGTTTGC XhoI
a

Underlining indicates sequence complementary to the chloramphenicol resistance gene; boldface indicates restriction enzyme sites.