FIG 3.

miRNA targeting regulates viral tropism in vivo and ameliorates cardiotoxicity and neurotoxicity. C57BL/6 mice were injected i.c. with Opti-MEM (n = 3) or 1 × 10⁵ TCID₅₀ of vMC₂₄-H (n = 5), vMC₂₄-N (n = 4), vMC₂₄-H2 (n = 4), vMC₂₄-C (n = 5), or vMC₂₄-NC (n = 5). Four days postinjection, all mice were euthanized and the infectious viral titers within the brain, spinal cord, and heart determined. Red symbols denote mice that developed HLP. The dotted line in brain tissue titration data represents the theoretical maximum input virus recovery and is based on the average brain weight of all mice analyzed. Individual viral titers are plotted along with the mean viral load (solid line).