FIG 2.

Distribution of RpK values among R. palustris genes. Read counts were normalized for total sample reads and gene length and tallied for the 5 to 90% length window of each gene to give reads-per-kilobase (RpK) values. Log₂-transformed RpK values were calculated and histograms were generated. The means and standard deviations of the distribution were calculated and used to determine 99.9% confidence intervals (indicated by shading). Genes with RpK values outside the confidence interval and below the mean are considered essential.