TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| V. cholerae strains | ||
| N16961 | O1 El Tor; VPI-1 VPI-2 VSP-I VSP-II Smr | 57 |
| O395 | O1 classical; VPI-1 VPI-2 | Laboratory strain |
| MO2 | O139; VPI-1 VPI-2 | Laboratory strain |
| SG-7 | Environmental | Laboratory strain |
| MC0817 (ΔvpiT) | N16961 ΔVC0817 Smr | This study |
| MC0847 (ΔintV1) | N16961 ΔVC0847 Smr | This study |
| CN1758 (ΔintV2) | N16961 ΔVC1758 Smr | This study |
| MC4758 (ΔintV1 ΔintV2) | ΔintV1 ΔVC1758 Smr | This study |
| ΔintV1 pintV1 strain | ΔintV1 strain harboring pBAintV1 | This study |
| ΔintV2 pintV2 strain | ΔintV2 strain harboring pBAintV2 | This study |
| SAM1785 (ΔvefA) | N16961 ΔVC1785 Smr | 43 |
| SAM1809 (ΔvefB) | N16961 ΔVC1809 Smr | 43 |
| MC0497 (ΔvefC) | N16961 ΔVC0497 Smr | This study |
| MC8509 (ΔvefA ΔvefB) | ΔvefA ΔVC1809 Smr | This study |
| MC850997 (ΔvefA ΔvefB ΔvefC) | ΔvefA ΔvefB ΔVC0497 Smr | This study |
| MC85099717 (ΔvefA ΔvefB ΔvefC ΔvpiT) | ΔvefA ΔvefB ΔvefC ΔVC0817 Smr | This study |
| ΔvefA ΔvefB ΔvefC pvefA strain | ΔvefA ΔvefB ΔvefC strain harboring pBBvefA | This study |
| ΔvefA vefB ΔvefC pvefB strain | ΔvefA ΔvefB ΔvefC strain harboring pBAvefB | This study |
| ΔvefA ΔvefB ΔvefC pvefC strain | ΔvefA ΔvefB ΔvefC strain harboring pBAvefC | This study |
| ΔvefA ΔvefB ΔvefC pintV2 strain | ΔvefA ΔvefB ΔvefC strain harboring pBAintV2 | This study |
| E. coli strains | ||
| DH5αλpir | Laboratory collection | |
| β2155λpir | Donor for bacterial conjugation, DAP auxotroph | Laboratory collection |
| BL21(DE3) | Expression strain | Laboratory collection |
| Plasmids | ||
| pJET1.2 | Cloning vector; Amr | Fermentas |
| pDS132 | Suicide vector; SacB Cmr | 58 |
| pBAD33 | Arabinose promoter; Cmr | 59 |
| pDSΔvpiT | pDS132 harboring truncated vpiT gene | This study |
| pDSΔintV1 | pDS132 harboring truncated intV1 gene | This study |
| pDSΔintV2 | pDS132 harboring truncated intV2 gene | This study |
| pDSΔvefA | pDS132 harboring truncated vefA gene | 43 |
| pDSΔvefB | pDS132 harboring truncated vefB gene | 43 |
| pDSΔvefC | pDS132 harboring truncated vefC gene | This study |
| pBAintV1 | pBAD33 with full copy of intV1 | This study |
| pBAintV2 | pBAD33 with full copy of intV2 | This study |
| pBAvefA | pBAD33 with full copy of vefA | This study |
| pBAvefB | pBAD33 with full copy of vefB | This study |
| pBAvefC | pBAD33 with full copy of vefC | This study |
| pMALc5x-His6x | Expression vector, TEV site; Amr | 60 |
| pMALc5x-His6x-VefA | pMALc5x-His6x harboring VefA | This study |
| pMALc5x-His6x-VefB | pMALc5x-His6x harboring VefB | This study |