TABLE 4.
Relative phosphorylating activities of His-RhaB from B. subtilis in the presence of various factorsa
| Reaction no. | His-RhaB | His-RhaA | Rhamnose | Rhamnulose | ATP | Mean relative activity (%) ± SDb |
|---|---|---|---|---|---|---|
| I | + | – | – | + | + | 100.0 ± 10.2 |
| II | + | – | + | – | + | 0.275 ± 0.389 |
| III | + | + | + | – | + | 29.2 ± 5.7 |
| IV | – | + | + | – | + | ND |
a
Each reaction was carried out at 30°C for 30 min in the presence (+) or absence (–) of 10 μg of His-RhaB, 1 μg of His-RhaA, 10 mM rhamnose, 10 mM rhamnulose, and 1 mM ATP in a total volume of 500 μl, and then an aliquot of the reaction mixture was taken for quantification of ADP generated as a by-product of phosphorylation as described in Materials and Methods.
b
The relative activities were based on the mean value of reaction mixture I (mean value ± the standard deviation in two independent experiments). ND, not detected.