FIG 1.

Characterization of L-domain mutant forms of LUJV Z. (A) Schematic representation of LASV Z, JUNV Z, TCRV Z, and WT and mutant forms of LUJV Z. The WT and all mutant forms of LUJV Z possess an HA tag at the C terminus. (B) VLP production driven by the WT and mutant forms was analyzed by WB. Actin (used as a loading control) was also detected (bottom). Normalized VLP production (VLPs per cell) is shown on the right. The data shown are averages and standard deviations of three independent experiments. (C) VLP production driven by the WT or mutant (YLCL-AAAA) protein was analyzed as described above. Actin (used as a loading control) was also detected (bottom). Normalized VLP production (VLPs per cell) is shown on the right. The data shown are averages and standard deviations of three independent experiments. (D) Inhibition of LCMV MG expression by LUJV Z and its mutant forms. BHK-21 cells were transfected with the expression plasmids for LCMV NP and L and with an LCMV MG containing the gene for mCherry together with the expression plasmid for LCMV Z, LUJV Z, or mutant LUJV Z. At 48 h posttransfection, cells were fixed and the numbers of mCherry-positive cells per microscopic field were automatically determined by BZ-X700 (Keyence). The data shown are averages and standard deviations of three independent experiments.