TABLE 1.
Primers used for UL18 mutant virus generationa
| Primer name | Sequence |
|---|---|
| Merlin-F | GACAACAGAGCTGAAGCATTCTGTACATCTTACGGGTTCTTTCCAGGGGAAATTAATATTACTTTTATCCATTACGGTCCTGTGACGGAAGATCACTTCG |
| Merlin-R | AAAAGATGGCTACGTAACATCCCTGATGGAAAGTCCCATCGAAGGTGGGAAGTAGCGGATTGCATTGAGGCTCGCTATCCTGAGGTTCTTATGGCTCTTG |
| AD169-F | GACAACAGAGCTGAAGCATTCTGTACATCTTACGGGTTCTTTCCAGGGGAAATTAATATTACTTTTATTCATTACGGTCCTGTGACGGAAGATCACTTCG |
| AD169-R | AAAAGATGGCTACGTAACATCCCTGATGGAAAGTCCCATCCAAGGTGGGAAGTAGCGGATTGCATTGAGGCTCGCTATCCTGAGGTTCTTATGGCTCTTG |
| Primer-F | AAACAAAACGTACATCGACGGTAA |
| Primer-R | AGCAAAGCGCATAAAAGCAGG |
a
Sequences used to generate AD169-UL18 Merlin mutant virus are designated Merlin-F (F, forward primer) and Merlin-R (R, reverse primer); those used to generate Merlin-UL18AD mutant virus are designated AD169-F and AD169-R. Primer-F and Primer-R were used to amplify the a3 domain of HCMV UL18 protein in a standard PCR.