FIG 1.

NPY deficiency is associated with increased neurological disease, as well as increased expression of monocyte-related mRNAs in the CNS. (A) Wild-type (129S1) and NPY^−/− (129S1 Npy^−/−) mice were infected within 24 h of birth with 10³ FFU of Fr98. The mice were then followed for clinical signs of neurological disease, including severe seizures and ataxia. The data are presented as percent nonclinical for 28 wild-type and 32 NPY^−/− mice. Statistical analysis was completed by Mantel-Cox analysis. P = 0.0013. (B) RNA was isolated from brain tissue of wild-type and NPY^−/− mice at 7, 10, 14, and 21 dpi and analyzed by real-time PCR for expression of viral RNA. The data are the means ± standard errors of the mean (SEM) for 3 to 6 samples per group per time point. Statistical analysis was completed by two-way analysis of variance (ANOVA) with Sidak's multiple-comparison test.