FIG 8.
NPY deficiency results in increased numbers of monocytes in the brain, but with phenotypes similar to those of monocytes from wild-type mice. (A and B) Brain tissue from wild-type and NPY−/− mice infected with Fr98 was removed at 3 weeks postinfection as described for Fig. 2. F4/80-positive cells were also gated on CD45 (y axes) by Ly6C (x axes) to identify M2 noninflammatory monocytes (upper left quadrants) and M1 inflammatory monocytes (upper right quadrants). The data are representative of multiple animals. Representative data from Fr98-infected wild-type (A) and NPY−/− (B) mice are shown. (C) The percentage of monocytes that were Ly6Chi was not statistically different in any group. The data are the means ± SEM for 4 mice per group and represent a minimum of two replicate experiments.