FIG 9.

Y2R expression on all three myeloid cell types in the CNS. (A) Microglia, M1 monocytes, and M2 monocytes were isolated by CD45 and Ly6C staining on CD11b⁺ Ly6G⁻ myeloid cells isolated from Fr98-infected wild-type mice at 3 weeks postinfection. The cells were analyzed for Y2R using directly conjugated antibodies. (B to E) Comparison of relative expression of Y2R on microglia (B), M1 monocytes (C), M2 monocytes (D), and all three cell populations (E). The gray line in each plot indicates no primary controls (antibody cocktail minus Y2R antibody).