FIG 3.
Real-time PCR of antiviral molecules downstream of the IFN receptor in BEAS-2B cells infected with PIV-3. BEAS-2B cells were infected with dolphin (T), bovine (B), or human (H) PIV-3 for 24, or 48, or 72 h. Cells treated with 20 μg/ml poly(I·C) and 0.6% Lipofectamine 3000 for 10 min were used as a positive control (+). Levels of downstream antiviral molecules (PKR, OAS, MxA, and ISG56) were measured by real-time PCR. The relative quantification (RQ) was calculated by normalization first to the GAPDH level to give a ΔCT value, followed by normalization to the mock level at a given time point (ΔΔCT). The RQ or fold change was then calculated as 2−ΔΔCT. A significant change in levels from those in uninfected controls at a particular time point is denoted as follows: *, P < 0.05; #, P < 0.01; +, P < 0.001.