FIG 5.

Regulation of EBNA2 binding by EBNA3A or EBNA3C at specific RBPJ sites. (A) EBNA3C, EBNA2, and RBPJ binding determined by ChIP-qPCR at the indicated loci in EBNA3C-HT LCLs cultured in the presence (4HT+) or absence (4HT−) of 4-hydroxytamoxifen for 2 weeks. The genomic loci included RBPJ binding sites near EBNA3C-repressed genes (BACH2, JAK1, and CXCR5 genes) or EBNA3A-repressed genes (HDAC7 and CDH1 genes), as indicated. The red dashed box indicates sites bound by EBNA3C from ChIP-seq data. All qPCR signals are reported as percentages of ChIPed DNA relative to input DNA. The results are shown as means ± SEM of three independent experiments. (B) Experiment analogous to that shown in panel A using the EBNA3A-HT LCL. EBNA3A, EBNA2, and RBPJ binding were determined by ChIP-qPCR at the indicated sites in the presence or absence of 4-hydroxytamoxifen. The red dashed box indicates sites bound by EBNA3A from ChIP-seq data.