Skip to main content
. 2016 Feb 26;90(6):3028–3043. doi: 10.1128/JVI.02936-15

FIG 2.

FIG 2

Detection of both EEHV2 and EEHV3 DNAs in lung nodules from two culled adult South African elephants by PCR amplification. Shown is agarose gel electrophoretic separation of ethidium bromide-stained first-round PCR products obtained by using EEHV2-specific POL primers LGH6525 and LGH7440 (500 bp) (lanes 1 to 4) and EEHV3- and EEHV4-specific TER primers LGH6707 and LGH6708 (310 bp) (lanes 5 to 7). Lanes: 1, AfLng47; 2, AfLng54; 3, Asian elephant EEHV1A case NAP11 heart; 4, negative-control African elephant vestibular lesion biopsy specimen; 5, AfLng47; 6, Asian elephant EEHV1A case NAP11 heart; 7, AfLng54. Three size marker lanes contain multimerized 123-bp ladders.