FIG 2.

Generation of rLCMVs containing synthetic S-like IGR sequences (Ssyns) in their S segments [rLCMV(IGR/Ssyn-L)]. (A) Predicted RNA secondary structures of Ssyn IGRs were determined using the CentroidFold web server (http://rtools.cbrc.jp). Each predicted base pair was colored using a heat color gradation from blue to red that corresponds to a base-pairing probability of from 0 to 1. (B) Growth kinetics of rLCMV(IGR/Ssyn-L). BHK-21 cells were infected (MOI = 0.01) with the rLCMV WT or the indicated rLCMV(IGR/Ssyn-L) construct. Virus titers in the TCS were determined at the indicated times p.i. Data represent the means ± SDs from three independent experiments.