FIG 5.

Generation of rLCMVs containing an altered L-IGR. (A) Growth kinetics of rLCMVs containing a synthetic S-like IGR in their L segment [rLCMV(IGR/S-Ssyn)]. BHK-21 cells were infected with the rLCMV WT or an rLCMV(IGR/S-Ssyn) construct (MOI = 0.01). Virus titers in the TCS were determined at the indicated times p.i. Data represent the means ± SDs from three independent experiments. (B) The predicted RNA secondary structures of genome (viral RNA [vRNA]) and antigenome (cRNA) S-IGRs of GGV were determined using the CentroidFold web server (http://rtools.cbrc.jp). Each predicted base pair was colored using a heat color gradation from blue to red that corresponds to a base-pairing probability of from 0 to 1. (C) Growth kinetics of rLCMV containing the GGV S-IGR in its L segment [rLCMV(IGR/S-Sggv)]. BHK-21 cells were infected with the rLCMV WT or rLCMV(IGR/S-Sggv) (MOI = 0.01). Virus titers in the TCS were determined at the indicated times p.i. Data represent the means ± SDs from three independent experiments.