FIG 1.

Characteristics of intestinal mucosal mast cells. (A) Enrichment and purification of mucosal mast cells from human healthy colorectal tissues. (B) Phenotype of purified mast cells as analyzed by immunostaining with specific antibodies and flow cytometry. (C) Intracellular immunostaining of tryptase (red) was confirmed by confocal microscopy; nuclei were stained with DAPI. DIC, differential interference contrast. (D) Positive staining of mast cells by toluidine blue. (E) Visualization of mast cells by transmission electron microscopy.