TABLE 1.
Primers and probes used for quantitative real-time PCR
| Target | Primer name | Primer sequence (5′–3′)a |
|---|---|---|
| HIV-1 integrants | Alu-F (1st cycle) | AGCCTCCCGAGTAGCTGGGA |
| SB704 (1st cycle) | TGCTGGGATTACAGGCGTGAG | |
| Gag-R (1st cycle) | CAATATCATACGCCGAGAGTGCGCGCTTCAGCAAG | |
| LTR-F (2nd cycle) | TTGTTACACCCTATGAGCCAGC | |
| Gag-R (2nd cycle) | CAATATCATACGCCGAGAGTGC | |
| P-HUS-SS1 (probe) | FAM-TAGTGTGTGCCCGTCTGTTGTGTGAC-TAMRA | |
| HIV-1 multiply spliced RNA | Tat-Rev-F | ATGGCAGGAAGAAGCGGAG |
| Tat-Rev-R | ATTCCTTCGGGCCTGTCG | |
| HIV-1 singly spliced RNA | Vpu-F | GGCGGCGACTGGAAGAAGC |
| Vpu-R | CTATGATTACTATGGACCACAC | |
| HIV-1 unspliced total RNA | Gag-F | GTGTGGAAAATCTCTAGCAGTGG |
| Gag-R | CGCTCTCGCACCCATCTC | |
| Late reverse transcription | MH531(F) | TGTGTGCCCGTCTGTTGTGT |
| MH532(R) | GAGTCCTGCGTCGAGAGATC | |
| LTR-P (probe) | FAM-CAGTGGCGCCCGAACAGGGA-TAMRA | |
| β-Actin | Actin-F | GGGAAATCGTGCGTGACAT |
| Actin-R | GTCAGGCAGCTCGTAGCTCTT |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.