Asyn expression does not alter WNV-induced TNF-α, IL-6, or interferon production. Brain tissue was harvested from the indicated regions at day 8 postinoculation with WNV (103 PFU s.c. footpad) to obtain tissue lysates. Following quantification of total protein concentrations, ELISAs for TNF-α (A), IL-6 (*, P = 0.04) (B), interferon alpha (C), and interferon beta (*, P = 0.05) (D) were completed. Data are for three replicates per treatment group, and the mean and SEM for each group are shown.