FIG 1.
Schematic of recombinant viruses. (A) BAC-derived TB40/E-mCherry was used to generate TB40/E-mCherry-US28-3×F (US28-3×F), which contains three tandem FLAG epitopes at the C terminus of the US28 ORF (54). TB40/E-mCherry-US28Δ (US28Δ) was generated by deleting the entire US28 epitope-tagged ORF (54). (B) To generate a recombinant in which US28 is the only intact HCMV GPCR ORF, we performed a series of recombination events whereby the entire coding regions for UL33, UL78, and US27 were excised and repaired by linear recombination. This resulted in a virus termed TB40/E-mCherry-US28wt (US28wt). Finally, using US28wt, we deleted US28 in its entirety, yielding TB40/E-mCherry-allΔ (allΔ) (54). TRL and TRS, long and short terminal repeat, respectively; UL and US, long and short unique region, respectively; IRL and IRS, long and short internal repeat, respectively.