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US28 is dispensable for lytic grown in fibroblasts. Primary fibroblasts (NuFF-1) were infected with the viruses indicated at a multiplicity of infection of 0.5 TCID50/cell (A) or 2.0 TCID50s/cell (B). Supernatants were harvested over a time course of 120 h, and then titers for each time point were determined by TCID50 assays. Samples were analyzed in triplicate.
