FIG 5.

US28Δ- and allΔ-infected Kasumi-3 cells result in IE1-positive infected cells. Kasumi-3 cells infected with each of the indicated viruses under latent conditions as described in the legend of Fig. 4 were harvested for immunofluorescence assay. Cells were stained with a monoclonal antibody directed at IE1 (clone 1B12) (56), shown in green. mCherry (red) is a marker of lytic infection; DAPI (blue) is shown as a nuclear marker. Images were collected using a 40× objective and depict representative fields for each infection.